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A stable, long-term 1,3-PDO production from glycerol was demonstrated with cells immobilized in a fibrous bed bioreactor operated in a repeated batch mode, which partially circumvented the 1,3-PDO inhibition problem.
The optimal strategy is shown to be one of the following: (1) a continuous-flow stirred tank reactor; (2) a repeated batch operation (spatial dual: a plug flow reactor with/without recycle); (3) a repeated fed-batch operatio involving singular control (spatial dual: a plug flow reactor with recycle and distributed feed); or a specific combination of (1), (2) and (3).
The fermentation was also studied in a fibrous bed bioreactor (FBB) operated in a repeated batch mode for 10 consecutive cycles in 10 days, achieving an average butanol yield of 0.21 ± 0.02 g/g and productivity of 0.53 ± 0.05 g/L·h from sugarcane juice, demonstrating its long-term stability without applying the antibiotic selection pressure.
The operating conditions were optimised (run time, 300 s; stop time, 3 s; power input, 6 W; harvest pump flow rate, 4 l per day) and a repeated batch process (three batches for a total of 192 h) was performed during which the SE was maintained always higher than 88%.
Furthermore, in the biofilm reactor, fermentations can be performed in a repeated batch mode or a continuous fermentation mode.
High biomass and lipid production were achieved in both batch and fed-batch cultures with glucose as carbon source, and a repeated batch process would be expected to be as efficient as a fed-batch or repeated fed-batch process.
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VFAs formed in all repeated batch fermentations, proving the suitability of the biocatalyst for continuous mode operation.
For the purpose of designing an automatic repeated batch ethanol fermentation process, a self-settlement feature of flocculating yeast was used along with the development of a relevant kinetic model to describe such an operation.
Moreover, high productivity of l-lactic acid in an open repeated batch fermentation system under non-sterile conditions was demonstrated.
Fermentation ability of the recombinant strain was successfully kept during a five-cycle repeated batch fermentation with 86.3% of theoretical yield based on starting biomass.
Furthermore, the cells can remain in the biofilm during a prolonged feeding fermentation or repeated batch fermentations, and the already established solvent-producing capacity of the cells would increase the carbon flux to solvent production rather than biomass synthesis.
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