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Manual tracing of the CC was performed by a single rater, BW, with a high intra-rater reliability (ICC >0.95 for repeated area measures).
This method allows a researcher to look for repeated areas of DNA on a molecule strand.
It yields highly reliable contigs and it is not so affected by repeated areas between contigs.
Gene duplications and repeated areas are prevalent in the SLY genome.
In genome data, there are many highly repeated areas, which are full of the same or similar sequences.
Repeated areas in the Giardia genome contain pseudogenes, retro-transposons and low complexity repeats and they could constitute areas of increased genomic plasticity or sites where recombination occurs, which is indicated by their associations with discontinued synteny.
Because Velvet and other de Bruijn graph-based programs cannot build across repeated areas (such as the inverted repeat regions in angiosperm plastomes), the plastome is assembled in segments, which need to be manually joined.
In order to assess health inequalities due to migrant status, gender and geographical area, a repeated cross-sectional study with secondary data-analysis was carried out in the time period 2006 2008 in Italy.
However, gaps can occur if reads that would typically be assembled into one contig cannot span a large repeat area.
Surprisingly, we got several amplification products of different sizes and the sequencing results showed that there might be an extensively high repeat area.
There are also CRISPR-associated (CAS) genes and a leader sequence before the repeat area.
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