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To dissect whether RAD51 or the BRC repeat serves as the actual target of IBR1/2, a sequential SPR binding assay was used to detect the inhibition of protein protein interaction.
The third helix of each repeat serves as 'recognition helix' facilitating intercalation of DNA in the major groove (Jia et al. 2004).
The gene is placed in a well-defined genomic context with an obvious ribosomal binding sequence GGGAGGG at -15 and two small inverted repeats serving as a putative rho-independent transcriptional terminator.
Manual refinement consisted in a method similar to ABRA [35] and Kajava's method [36], with knowledge about the approximate location and number of repeats serving to identify the true repeat boundaries.
An electro-positive groove formed by tandem ARM repeats serves as a binding site for the JMD and CBD, which can be postranslationally modified at multiple sites; depending on the site, the interaction between cadherin and catenins is either strengthened or weakened (Dupre-Crochet et al., 2007).
While bearing the findings of IS1E transposition into both the promoter region and the repeats array, we are unable to distinguish if the AATATT triplet repeats serve as usual hotspots of transpositions, or does it do so only in the repeats transcription?
Rather, the repeats serve as candidates for PCR primer design.
Leucine-rich repeats serve as recognition motifs for surface proteins in bacteria and eukaryotes.
This finding suggests once again that these particular hexameric repeats served as microhomologies.
Specifically, some repeats serve as building blocks in superstructure and thus steric constraints limit the expansion of the repeated units [ 11].
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