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In addition to the FASII pathway, a regulation of three genes encoding lipases (EF0169, EF1683 and EF3191) and two genes encoding cardiolipin synthetases (EF0631 and EF1608) further indicates both degradation and processing of fatty acids (Table S1).
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Similarly, we observed in the present study a positive regulation of three genes (sodC, katA, ahpC) involved in the protection against oxidative stress.
We identified a comparative up regulation of three biological processes generation of precursor metabolites and energy, transport, and cell communication in cumulus cells compared to oocytes.
From GO associations of differentially expressed proteins we found that there was a comparative up regulation of three biological processes in high fertility spermatozoa: metabolism, cell communication and cell motility (Fig 2).
A general consensus indicates that such switches are based on a mutual regulation of two transcription factors (figure 1), e.g. mutual inhibition: protein A inhibits the synthesis of protein B and vice versa [12].
This resulted in a down regulation of two wnt target genes, LEF and cyclinD1 and the wnt pathway reporter.
There was also a down regulation of two important downstream effectors of the wnt pathway, namely LEF and cyclin D1 genes.
Similarly, Ribarits et al. (2007) found a differential regulation of two ProDH isoforms during dehydration in tobacco, while under these conditions the Arabidopsis ProDHs were co-regulated [ 23].
Similar analysis of necroinflammatory disease metabolites indicated a relative up regulation of six amino acids, three dicarboxylic acids and one tricarboxylic acid (Table 2, Additional file 1: Figure S1).
The most striking result is that AMLs bearing the t(15 17) translocation have a distinctive up regulation of seven miRNAs located on the human 14q32 imprinted domain.
In addition, our study revealed a temporal differential regulation of five miRNAs bta-miR-193a-3p bta-miR-193a-3p bta-miR-193a-3p bta-miR-193a-3p) in unchallenged cells.
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