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We used a dataset recently published by Roepman et al. as reference dataset and an independent gene expression dataset of 11 metastasized and 11 non-metastasized HNSCC tumors as validation dataset.
Ground survey data collected in June 2013 within the study area were treated as a reference dataset for lidar DTM validation.
The cut-off points for dividing the EuroGenomics data into the reference dataset and the validation dataset differed between the four partners in order to meet the requirement that the size of the validation data should be about 25% of that of the national dataset.
23 significant differentially expressed pathways were found in the reference dataset, and 10 in the validation dataset, 7 of which overlapped.
Next, in analogy with the study by Roepman et al., we performed LIMMA analysis on the samples stored after 1998 only (66 samples in the reference dataset and 8 in the validation dataset) [ 3].
Overall map accuracy was 75.7% and Cohens Kappa was 0.725 (Table 3), suggesting the strength of agreement between the independent validation (i.e., reference) dataset and classification to be good to very good42,43.
The available two-breed dataset was partitioned into a reference (or calibration) and a validation population based on sex.
In addition, we also provided a valuable reference dataset for future identification and validation of biomarkers for detection, diagnosis and prognosis of OTSCC.
Acquired measurements constitute an accurate ionospheric reference dataset for validation of the variety of models and alternative measurement techniques.
GitHub serves our open-source code, reference datasets, and the resulting multi-dimensional distributions that define most validation criteria.
It achieved similar or better prediction accuracy than the known methods aforementioned for most of the tested datasets in our validation process.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com