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The phrase "a reference category against" is correct and usable in written English.
It is typically used in statistical or analytical contexts to denote a baseline or comparison group in relation to other categories.
Example: "In this study, we will use the control group as a reference category against which we will compare the experimental results."
Alternatives: "a baseline category for" or "a comparison category to".
Exact(1)
Table 4 presents associations of demographic characteristics, health behaviours and work characteristics with occupational sitting using categories of low, moderate and high occupational sitting (with low occupational sitting used as a reference category against moderate and high sitting times) separately for men (n =1945) and women (n = 2491).
Similar(59)
a: reference category.
Here a reference category (Ref).
Excellent SRH was used a reference category.
a Reference category: no aural symptoms.
a Reference category: Good physical health.
this group served as a reference category.
a The genotype served as reference category a The genotype served as reference category a The genotype served as a reference category.
a Reference category is no history of hypertension.
Normal BMI was used as a reference category.
Gender was coded with men as a reference category.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com