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The clear supernatant was collected and absorbance measured against a reference blank of distilled water at 532 nm.
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Background correction was made using a reference blank KBr pellet.
The reference blank contained all of the above reagents, and buffer instead of AR, to correct for nonspecific reduction of NADPH.
This measurement is taken as the reference blank measurement and compared with a duplicate measurement of the sample extract.
After incubation, potassium iodide solution (100 μL; 20 mmol/L) was added and absorption was measured against reference blank cuvette (100 μL PBS instead of extract; absorbance corresponding to 100% HOCl) at 350 nm.
A clear solvent solution serves as the reference blank.
The absorbance of clear supernatant was measured against reference blank at 535 nm.
The reference blank contained the TBA reagent.
As a reference, 100 μl of blank medium containing 3000 ng of bacterial collagenase was added to one set of wells for complete digestion of FITC collagen.
Medium containing 500 µg/ml FITC-inulin was used as a reference of the initial experimental conditions, and medium without FITC-inulin as blank.
Blank analysis of the HILIC column showed slightly negative values compared with a reference of pure PBS.
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CEO of Professional Science Editing for Scientists @ prosciediting.com