Since the cytoplasmic compartment lacks chaperones assisting in protein folding and does not provide a sufficient reducing milieu for disulfide bond formation, folding and dimerization of recombinant vIL-10 proteins is unlikely to occur in the bacterial cytoplasm [ 15].
This spacer is designed to be cleaved in the reducing milieu of the cytoplasm and thus decrease lipid toxicity.
We selected methylene blue (MB) as this antimalarial was shown to challenge the parasites intracellular reducing milieu through the generation of pro-oxidant H2O2 [17].
Although others have conferred the beneficial effects of intracellular reducing environment to glutathione only [46], [49], [50], the possible implication of the reducing milieu in its whole on transfection efficiency of bioreducible transfectants has been overlooked.
The characters in "Rappaccini's Daughter" are much given to exclaiming "Perchance" or "Methinks", but the stagey vocalising, far from reducing the milieu into a poor pseudo-Scottian pastiche, actually gives them a strange floating temporality, halfway between myth and social reality.
Although the bone marrow texture is distored by fibrosis, donor-stem cells can engraft after a reduced intensity conditioning without obvious delay, suggesting that the bone marrow milieu for stem cell homing is apparently not seriously altered.
In the plant, PAs usually occur as N-oxides, which are easily converted to their respective free base in the reducing gut milieu of any herbivorous vertebrate or insect feeding on these plants [21], [22].
To circumvent the complex protein milieu of plasma and discover robust predictive biomarkers for preeclampsia (PE), we investigate if phospholipid-binding ligands can reduce the milieu complexity by extracting plasma extracellular vesicles for biomarker discovery.
Multiple actions and approaches within a pluralist milieu reduce politics to a market where momentary initiatives compete with each other for funding and attention.
This would be a sign of a rather inflammatory milieu, but in contrast, such a hypoxic milieu reduces the cytotoxicity of CTLs, which is simulated by the normoxic cell culture supernatant.
