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Introduction of G18A and U54C mutations, which disrupts the interaction between the D and TΨC loops, reduced label transfer efficiency.
Immunofluorescence analysis of MADA fibroblasts at passage 25 showed a markedly reduced labelling by 1188-2 antibody (Fig. 1b).
There was a trend towards reduced labelling for cyclin D1 in the NVP-BEZ235-treated groups, and this was statistically significant in OCIP17, 19, and 21, whereas in the non-responsive OCIP18 model there was a non-significant increase in cyclin D1 in the NVP-BEZ235-treated animals.
Labeling one day earlier (E4.5) marks a similar distribution of cells but with reduced labeling.
In both BICD2-N and G1del mice, changes in endosomal labelling were most evident in sections stained for GRASP-1, as reduced labelling was associated with a loss of the relatively large particles.
However, the reduction of the PMT voltage, power of the lasers, or reduced labeling, does not address the question of whether or not a particular cDNA on a microarray is biologically saturated.
There was no requirement for it to do so because of reduced labelling requirements for food produced on-site.
In special cases, reduced labeling degree of the tracer substrate can increase the precision of flux analysis.
Reduced labelling requirements for food produced on site meant it was sufficient for general allergen warnings to be posted around the shop, instructing customers to consult staff for advice.
24-hr exposure to MβCD visibly reduced labeling intensity.
Tissue incubated with aldosterone and the antibody MA1-620 had the appearance of reduced labeling.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com