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Using the filter provides a reduced background in the low energy region and eliminates, for instance, the Rh-L and Ar-K lines originating from the tube and the air (Additional file 1: Figure S1).
Furthermore, the enhanced filter and lighting configuration ensures a reduced background signal, aiding the capture of high-contrast images that are ideal for publication purposes and for working in preparative modes.
Thus, the incorporation of ligation allowed for easy elimination of interferences from coexisting DNA and a reduced background.
Future evaluation of backcrossed lines with a reduced background mutation load will help clarify the effect of SBEIIa mutations on both starch content and seed weight.
As shown in Figure 3, with high efficiency and high fidelity of EcoRI, the enzymatic cleavage reaction only occurs on those probes that retain their stem loop structure without capturing the target, leading to a reduced background signal.
The smoke was considered to be toxic when reduced growth of the bacteria (smaller number and/or smaller colonies) on the selective agar plates occurred, or when a reduced background lawn was observed.
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We observed a strongly reduced background amplification in case only MseI was used in VIDISCA (Figure 2, dots all indicate viral fragments).
Indeed, a direct comparison of Ptet-14, Ptet-T3 and Ptet-T4 with Ptet-1 by transfection, revealed a strongly reduced background expression in the off-state for these three promoters (10-, 30-and 40-fold, respectively).
This is a clear example of the type of additional information that may be obtained when using a strain with reduced background fluorescence, thus revealing novel biological data.
Wang et al. engineered a synthetic chimeric protein, termed GAVPO, which consists of a Gal4 DNA-binding domain, VVD with point mutations for reduced background activity in a dark state and a p65 transactivation domain (Wang et al., 2012 ).
Importantly, a magnetic graphene oxide (MGO) is introduced to remarkably reduced background, which plays an important role in enhancing the signal-to-noise ratio and improving the detection sensitivity.
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