When purified Alr4641 was added to MDH, a marked decrease in the scattering of light was observed indicating reduced aggregation of MDH.
Finally, we provide a possible structural explanation for the reduced aggregation rate of Aβ fibrils containing the Flemish disease-causing mutation.
Compared to reduced Aβ42, the absence of a second β-hairpin in Aβ40 and the sampling of alternate β topologies by M35-oxidized Aβ42 may explain the reduced aggregation rates of these forms.
In the case of Aβ (1 40) peptides, only small oligomeric species were detected, in agreement with the reduced aggregation propensities of these species.
The current cropping system of conventional tillage and stubble removal in the northwestern Loess Plateau of China is known to decrease the water use efficiency and crop yield because of reduced aggregation and aggregate stability, as well as degrading other soil properties.
Numerous examples of other amyloidogenic proteins capable of aggregation individually, but showing a reduced rate of aggregation when combined in the same reaction, have been reported including prion proteins from different species [27] and different Aβ peptides [28].
Although the protein can be produced in Escherichia coli in a non-glycosylated form, the addition of glycans confers a reduced rate of aggregation as well as a 10-fold higher bioactivity.
In contrast, a combination of prednisolone with MRS2395 reduced aggregation to 22±3%3% (P < 0.05 when compared to either inhibitor used alone) (Fig. 4C).
Indeed, despite the overwhelming evidence that α-syn is a disordered monomer in solution, two recent reports suggest that the native protein exists as a helical tetramer under physiological conditions, with reduced aggregation tendencies, and that the dissociation of the tetramer into monomeric subunits promotes toxic aggregation [ 187, 188].
By using cell-based aggregation assays, we identified several novel variants of SOD1G93A with reduced aggregation propensity within mammalian cells.
