Sentence examples for a ready-to-use solution from inspiring English sources

After three further washings, the coverslips were air-dried and mounted with DAPI (4',6-diamidino-2-phenylindole) from a ready-to-use solution, ProLong Gold Antifade Reagent (Molecular Probes).

In order to determine CRNK-16 vitality, cells were stained with 7-amino-actinomycin D (7-AAD), a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis (for details see Schmid et al. [49]).

For direct fluorescence with DAPI (4',6'-diamidino-2-phenylindole) staining, cells were covered with 5 µM DAPI from a ready-to-use solution, "ProLong Gold Antifade Reagent" (Molecular Probes) and stained for 10 min in the dark prior to observation.

The WST-1 reagent is a ready-to-use solution which was added to the cells at a concentration of 100  μL/mL.

The TRIzol® LS reagent used herein is a ready-to-use solution for isolation of total RNA from liquid samples of different origins (TRIzol® LS).

Prolutex was provided to the patient as multiple vials each containing 25 mg of lyophilized progesterone and a 1-ml syringe of sterile water for solubilization (now available as a ready-to-use solution in some European countries), whereas Endometrin was supplied as vaginal inserts with vaginal applicators in individually sealed foil pouches.

In brief, PCR was performed in 40 μl volumes containing 4 μl of isolated DNA sample and 36 μl of an optimized ready-to-use solution of MasterMix at the following conditions: UNG was activated for 2 min at 37 °C followed by 15 min at 96 °C of DNA denaturation.

Sterile filter the solution into a glass bottle of at least 150 ml and keep the ready-to-use solution warmed at 37 °C in order to avoid any loss of activity (for details, see section " Pitfalls and limitations in the isolation of primary human hepatocytes ").

Slides were then rinsed with tap water, air dried at room temperature, overlaid with DAPI (4', 6'-diamidino-2-phenylindole) ready-to-use solution (Molecular Probes, Montluçon, France) and examined using a Leica DM 2500 upright fluorescence microscope at 100× magnification with a FITC-rhodamine double band filter.

DOI: 10.1038/sj/bjc/6600094 www.bjcancer.com © 2002 Cancer Research UK In these previous studies we provided a simple method to label Caelyx by incubation of 5 mg of the ready-to-use solution with 20 mCi of 99mTc-DTPA.

After the final wash step, ABTS-substrate (Sigma, ready-to-use solution) was added and the optical density was determined at 405 nm.