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Therefore, the anticipatory time was divided into a reaction time-dependent anticipation period (AT, like in Study 1), with a value of 1800 msec minus RT, and a reaction time-independent anticipation period, i.e., parametric AT PATT), with values of 0, 500, and 1000 msec.
Under our optimized conditions, addition of MdmX produced ~8-fold increase in FRET signals in an MdmX concentration-dependent manner and reaction time-dependent manner.
Thus, our observed distinct time-dependent inhibition of β-hydroxymethyl chalcone toward HDAC2 can be attributed to the "tandem reaction time-dependent tight-binding kinetics" mechanism, while such a time-dependent inhibition effect is much smaller for β-hydroxymethyl chalcone in HDAC1, and no time-dependent effect is observed for β-aminomethyl chalcone toward either HDAC1 or HDAC2.
Detailed reaction time-dependent morphological and component evolution was studied to understand the growth process of Mn3O4 nanorods.
This leads to tPA-concentration and reaction time-dependent changes in the surface properties, which is reflected by coinciding changes in colloidal stability.
We clearly demonstrated the reaction efficacy and the concentration and reaction time-dependent influence on tPA binding on the hydrodynamic size and surface characteristics of the particles using DLS and pH-dependent electrokinetic mobility assays.
Similar reaction time-dependent profiles of SERS were observed for the Ag/GL substrates prepared in both lower (1/0.5 mM) and higher (10/5 mM) concentrations of AgNO3/BuNH2 solutions (Figure 5A,C).
Here, we suggest the subsequent dynamic equilibrium of the intramolecular nucleophilic attack reaction (namely the formation of [EI]′ complex) as another contributing factor (D) to account for the time-dependent inhibition phenomenon, which can be termed as the "tandem reaction time-dependent tight-binding kinetics" mechanism (see Figure 4).
Figure 3b shows that this effect is also reaction-time dependent.
Having a quick reaction time is dependent on being keenly aware of what's coming.
Three NaOH dosages of 6.0 mM (corresponding to a low pH), 7.8 mM (a medium pH, near the optimal condition), and 9.0 mM (a high pH) were used to prepare reaction-time-dependent samples under different pH conditions.
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