Even in golf, a sport whose pace lends itself to lyrical, thoughtful commentary, sometimes an instant reaction is required, and when a brilliant shot is played then the heat is on to come up with something apposite.
In practice, an almost immediate reaction is required when unexpected events occur, meaning that trains must be rescheduled in a matter of seconds.
A kinetic rate expression for this reaction is required for design and modeling of a microreactor for the DC process.
Since only a small portion of the reaction is required, without any intermediate purification step, the time required for this analysis usually fits between typical ligation and transformation procedures.
An RS domain tethered to the ESE position was found to be insufficient to activate splicing of an IgM substrate in S100 complementation assays, and the addition of an intact SR protein to the reaction is required for splicing to occur under these conditions [27], [45], [59].
Because ATRA is tolerant of the investigated functional groups, no protection/deprotection reaction is required, which is a major advantage of the method.
Although the amplification reaction of NASBA is isothermal, a single melting step prior to the amplification reaction is required to allow annealing of the primers to the target, which is not required in LAMP [35, 36].
In addition, the reaction entropy ΔS r for the garnet-forming reaction is required to estimate the extent to which the Gibbs free energy ΔG r was overstepped.
