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To demonstrate this potential, we applied a rapid lifetime determination technique, using only two time-gated fluorescence images to produce each lifetime image [ 33].
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For rapid lifetime determination on a Victor 2 reader (PerkinElmer), the "time-resolved fluorescence" mode was used, with D340 excitation and D642 emission filters, measuring at two delay times (t1=30 μs and t2=40 μs) with a gate time of 100 μs and a total counting cycle of 1 s.
The sensor luminescence was optically filtered with a long-pass filter (RG630, Fa. Schott, Mainz, Germany) and detected via rapid lifetime determination (RLD) (for details see, Liebsch et al, 2000, 2001).
The method for rapid lifetime determination FLIM has previously been described in detail, see reference [ 33] and references therein.
However, reliable sensing of O2 on such instruments by using the rapid lifetime determination (RLD) method 41 was still possible over the O2 range 0 250 μ m (0 21 % atmospheric O2).
Here a frequency-domain response model is coupled with a pseudo-asymptotic integration scheme to provide a rapid estimate of lifetime reliability and hence the extreme response at a design target level.
We've seen in our lifetimes a rapid evolution in the wearables family — it has brought together four core branches of technology into a new class of device.
Looking for potential candidates to progressive accumulation of damage over a lifetime, it seems reasonable to exclude RNA, proteins and other cellular macromolecules with a rapid turned over.
We show a rapid, brassinolide-induced cell wall expansion and a fast BR-regulated change in the BRI1-GFP fluorescence lifetime in the plasmamembrane in vivo.
I was a "Rapid" fan.
Bring to a rapid simmer.
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