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Due to differences from the target species, such as sequence divergence, non-target transcripts will exhibit a range of probe hybridization efficiencies that cause technical variation in hybridization intensities.
Figure 3 illustrates the performance curves for Splitter with and without normalization over a range of probe intensity threshold values (see Methods).
A range of probe dependencies have been observed for synthetic CCR5 ligands: from compounds that block chemokine binding but not HIV-1 gp-120 binding to compounds that block HIV-1 binding but partially spare CCR5 function through chemokine signaling.
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The mounting device has been used to hold a range of probes in a number of different animal models of intensive care including studies of brain death, traumatic brain injury, and sepsis.
Activity coefficients and interaction parameters for a range of probes have been measured and allow us to determine the nature and origin of the thermodynamic interactions in the systems.
The cognitive interview will consist of an investigator accompanying the participants as they complete the AHP task and asking a range of probing questions.
We have now completed many exhaustive attempts at detecting a GM-CSF siRNA in the silenced lines using various T cell preparations at different stages of blast cell transformation, using a range of probes and have failed to find such an RNA anywhere in the GM-CSF locus (data not shown).
Since most bacterial species exhibit a range of variability, probe specificity is inevitably limited.
The apparent Km (Michaelis constant) and Vmax (maximum reaction velocity) values were determined in a range of concentrations of probe drugs.
The Ki values were determined in a range of concentrations of probe substrates (approximately Km/2, Km, 2Km and 4Km) and different concentrations of EB (0, 100, 200, 300 μM) and EE 0, 100, 200, 300 μM).
These sampling strategies are applied to whole three-dimensional organs, well-defined fragments of organs, and ultimately to two-dimensional sections interrogated using a range of stereological probes including points, lines, curves, frames, and optical and physical disectors.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com