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Prior to the arrival of NGS platforms, WGS on larger genomes was possible because Sanger-based sequencing platforms generated paired-end reads of 700 bp or greater and a range of insert sizes from 3 to 40 kb (Weber and Myers 1997).
Based on the age and diversity of pine repeats, it may even be possible to assemble the genome using a whole genome shotgun strategy based on several platforms and a range of insert sizes.
The metagenomic libraries consisted of a total of 23,872 clones: 7,744 from SRF, 8,448 from SAB and 7,680 from LAB, with a range of insert sizes of 30 35 kbp.
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Using the robust Taq enzyme or a proofreading Pfu DNA polymerase mutant, the method is applicable to a broad range of insert sequences.
She had launched Sizers, a range of shoe-size inserts, in 2013 after struggling to find shoes that fit for a last-minute job interview.
We conclude that GFP-based FRET can be useful for studying intrinsically unstructured proteins, and we present a range of calibrated protein inserts to experimentally determine the distances that can be studied.
We also present here a large set of in vitro TEER data recorded for each endothelial cell line cultivated on a range of tissue culture inserts from different manufacturers, with the aid of a CellZscope on-line TEER recording system [ 30].
The structure of the complex is modified in some insects, Drosophila being an example, where two bHLH-orange domain genes are absent from the complex and there are seven copies of the remaining one, the Brd-class gene, mα, has been duplicated and there are a range of unrelated genes inserted in the complex [ 6, 9– 16].
Due to a newly designed type of polylinker, directional cloning of a wide range of inserts is greatly facilitated, and HA-tagging of the insert of interest is possible if needed, depending upon which pPRIG vector is selected.
And, in addition to that, the host expression machinery might be tuned to a broad range of inserts, with varying fragment types, origins and sizes.
In only a relatively short period of time, a range of transgenes have been inserted into more than 10 insect pest species.
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