The melt temperature, with a range of 1.5°C either side of predicted Tm, was deemed acceptable if the melt curve was normal sigmoidal.
A range of concentrations of either class B or class R INH-ODNs were added simultaneously.
In the wells of a standard black 96-well microplate, solutions were prepared with a fixed concentration of APTS-MT (100 nM) and a range of concentrations of either unmodified or PEGylated ConA (4 nM to 10 μM).
Cytotoxicity was measured using a Sulphorhodamine B assay and clonogenic survival following exposure of six different pancreatic cancer cell lines to a range of doses of either gemcitabine, AR-A014418 or both for 24, 48 and 72 h.
The assay was performed as described [ 12] using a range of dilutions of either spiradoline or dynorphin A 1 13 peptide (Sigma).
Using the SRB cell proliferation assay, the growth of BxPC-3 and MIA PaCa-2 cell lines was measured after 24, 48, and 72 h of exposure to a range of concentrations of either AR-18, gemcitabine, or a concurrent combination of both drugs; using either a fixed ratio of 200 1 AR-18 to gemcitabine, or variable doses of both drugs.
In most contexts, a range of gestures of either type was used, and only for nursing and travel were gestures exclusively ones that might have derived from ontogenetic ritualization.
Silastic capsules containing a range of doses of either ss-hexachlorocyclohexane (ss-HCH) or o, p'-dichlorodiphenyltrichloroethane (o,p'-DDT) were implanted subcutaneously, and animals were killed after 1 week.
Prior exposure to a dose range of either BPA or MP resulted in a dramatic, concentration-dependent complete to partial evasion from the G1-phase arrest induced by 10 μM OHT and a concurrent increase in the S-phase fraction.
