99.7% (624/626) of Nm isolates tested were sodC-positive, with a range of average Ct values from 13.0 to 29.5.
The 12 RGs investigated displayed a wide range of Ct values in separate evaluation of the paired samples.
Although just a few samples were used in this study, the robustness of the assay was demonstrated consistently for all tested assays varying at a wide range of Ct values.
The 12 RGs displayed showed a wide range of Ct values: except for rRNA18S (mean 9.8), the mean values of all the commercial RGs and ESD ranged from 19 to 26, whereas those of the microarray-selected RGs (BTF-3, YAP1, HIST1H2BC, RPL30) exceeded 26.
qRT-PCR of a housekeeping gene proved very informative and was usable for quality assurance of each sample by defining an acceptable range of Ct scores.
Hurwitz et al. [21] used an anthropomorphic phantom to determine fetal doses from a range of multidetector-row CT investigations in the early stages of pregnancy.
Overall, the MBS setting supported a more diverse range of CT services to a younger, more female patient base in greater volume than the public hospital setting.
About half or more of the county councils offered an additional range of CTs, provided in various ways by diverse conventional and CT practitioners [ 17].
Before relative quantification, C9orf72 gene was subjected to a serial dilution assay to determine the optimum detection range of Ct values, with a Ct threshold of 35 for undetectable mRNA levels of expression.
