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Infection was quantified by using a range of assay read-outs that included extracellular or intracellular p24 antigen detection, RNA quantification and luciferase and beta-galactosidase reporter gene expression.
The robustness of the assay conditions was tested in a range of assay parameters near the optimal conditions.
This validation by a range of assay methods provides not only confirmation of positivity but also differentiation between vaccination and infection.
If possible, samples should be collected in a way that will allow as wide a range of assay types as can be predicted.
Application of different techniques can validate data obtained by one method by the results of the others, yield more specific information on a particular aspect of a system, and provide results in a range of assay conditions ordinarily not available to a single method.
Similar(55)
We have used a range of assays in order to establish that CysC enhances autophagy in neuronal cells under toxic conditions as a protective mechanism.
This was assessed using a range of assays.
We ran 3,745 PCRs, covering a range of assays, to increase detection sensitivity.
A range of assays were used to measure HCV RNA levels at local investigational sites.
During trial visits, which were scheduled approximately every 6 months, patients underwent a range of assays including a one-off deposition scan.
With studies of ion channels, it is helpful to have a potent and specific blocker that is easy to use and applicable to a range of assays.
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