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The optimized method displayed a quantitative range of 375 4,500 pg/mL.
Amplification was linear and efficient for all targets within the quantitative range 60 6 000 000 gene copies/reaction, with the exception of the gyrB target, which had a quantitative range of 600 6 000 000 gene copies/reaction (Table 4).
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Eleven transcripts were selected based primarily on encompassing a quantitative range indicative of transcriptional factors, which for this group of targets was estimated to be 10 10,000 transcript molecules per 10 ng of total RNA.
Gene expression was analyzed using a custom, high-density DNA microarray (a single nucleotide tiling array) with a precise and wide quantitative range of mRNA concentrations [ 26, 27], as previously described for genome resequencing [ 28] and transcriptome analysis [ 29].
The observation that those genes expressed at low levels had the lowest intra-individual correlations may be partly due to the known characteristic of probe sets at the lower end of the quantitative range of a microarray to have poorer reproducibility [ 36].
In comparison, URM students admitted in 2011 2012 had a quantitative score range of 410 760 among a matriculating pool with a range from 470 to 800.
The assays showed a linear quantitative range over five orders of magnitude from 2×105 down to 2×100 genomes per reaction, with linear regression equations of Cp = −3.37 log (genome number).
The RTD-PCR produced a linear quantitative detection range of 7 logs with a lower detection limit of 10 CFU/g tissue.
RTD-PCR produced a linear quantitative detection range of 7 logs with a lower detection limit of 10 CFU/g tissue, or a few copies per reaction (Table 1).
This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 10 colony-forming units (CFU /g tissue or a few copies per reaction.
Depending on the quantity and quality and relevance of the data available, such data can be considered in a qualitative way or in a quantitative way, ranging from a listing of the costs to a modelling exercise [ 24, 25].
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