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Five patients had a quantitative decrease in expression of ER positivity between primary tumor and metastasis, of whom three became ER negative by the above definition.
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The mutations conferring IP in general cause a quantitative decrease in the expression or a partial loss of the RB1 suppressor function [ 3, 10, 17, 24].
This study has demonstrated a decrease in expression of P-gp and down regulation of MDR-1 gene consistently by flow cytometry and quantitative RT-PCR, but an increased expression of type II collagen on MSC during chondrogenesis.
For example, Ap4 deficiency resulted in a decrease in expression of the transcription factor Spdef.
Analysis of endogenous Pmp22 expression levels by quantitative RT-PCR revealed a similar decrease in expression.
We recently suggested that a subtle decrease in the expression of one allele of the TGFBR1 gene was a heritable quantitative trait predisposing to CRC.
a quantitative analysis results of expression of CGRP in different groups.
An increase in the expression of E-cadherin and a decrease in the expression of vimentin were seen by quantitative RT-PCR (Fig. 6a) and Western blotting (Fig. 6b) after Smo siRNA treatment in GBd15 and TGBC2TKB cells.
The array data as well as validation in A549 and HeLa cells treated with BrdU for 48 h by quantitative PCR confirmed the increase in expression of CXCR4 receptor (Fig. 1b) and decrease in proliferation as expected (Supplementary Fig S1b).
(C) Semi-quantitative RT-PCR analysis showed marked decrease in the expression of IFN-α and β following STING knock-down after JEV infection.
We used quantitative RT-PCR to examine quantitative changes in the expression of CDKN1C.
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