Similarly to the standard real-time PCR platform, a linear quantification range of 6 orders of magnitude was achieved with a sensitivity of 103 P. infestans target copies/μl initial ligation mixture.
As shown in figure 2B, a linear quantification range of 6 orders of magnitude was achieved with a sensitivity of 103 P. infestans target copies/μl initial ligation mixture.
The assay showed a wide quantification range of 5 log units using calibrated cell suspensions of Pediococcus parvulus 2.6 and Oenococcus oeni I4.
We achieved a linear quantification range of 200 to 5,000 pg/mL for ascites samples.
U1A has a linear quantification range of 10 to 10 molecules (R = 0.99, p < 0.001, fig 2e).
As the samples varied in size, two RNA isolation kits were applied that each were specially designed to cover a certain quantification range of cells.
When GE-maize cultivars MON81 and NK603 were tested with real-time PCR [ 54], a quantification range from 0.5%to100%0% in 100 ng of non-GE DNA was reported.
The former test had a detection limit of 103 cDNA molecules per reaction with a log-linear quantification range of up to 1011 (R2 = 0.98), while the latter test was able to detect 500 cDNA-BTV molecules/PCR.
This technique is high throughput-oriented [ 18, 19] and offers a wide dynamic quantification range of up to four orders of magnitude with high analytical sensitivity and specificity [ 20, 21].
A high background signal for blank control samples containing no analyte worsens the limits of detection and quantification for real samples and limits the linear quantification range of an assay.
