In this paper we propose an alternative novel approach which takes into account quantifiable expression levels of all genes assayed.
A 25 μl qPCR consisting of 25 ng diluted cDNA, QuantiFast® Sybr Green Dye (Qiagen) and 0.1 μM of each qPCR primer pair was performed to obtain quantifiable expressions of Wnt and EMT-related gene targets in MCF7 and TamR cells.
As both the samples contained RNA extracted from tissues of approximately 35 mosquitoes each and pooled to form one single sample, a quantifiable estimation of gene expression was expected with a minimum chance of aberrations.
mRNA (figure 1E) and western blotting (figure 1F,G) confirmed a quantifiable increase in netrin-1 expression in the whole colon (figure 1E,F) and in the mucosal lining layer (figure 1G) following 7 days of DSS.
A trade secret can be a quantifiable design, composition, formula or pattern.
The levels of endogenous fluorescence would be a quantifiable measure of how much the expression levels are changes and this could provide an alternative measure to the qRT-PCR data.
And that could trigger a quantifiable improvement.
Only scenes that produce a quantifiable rise in blood pressure.
One wants emotion and range and a quantifiable physical transformation.
