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Benzylthiol-functionalized gold nanoparticles were synthesized by a modified two-phase method, in which a purification procedure improved the structural and morphological quality of the gold nanoparticles.
After a purification procedure the sample of chitosan was dissolved using 0.05 M HCl and it was stirred for 18 h at room temperature.
Although some attempts have been made to destroy the organo-mineral associations before HS extraction [13],[14], a purification procedure aimed at obtaining ash-free organic fractions is commonly carried out on the extracted humic materials.
A purification procedure, based on thermal treatments in strong acid solutions, was used to remove inorganic and organic impurities from diatomite and to make them a safe material for medical applications.
To enable biophysical studies of Bqt1 and Bqt2, we established a purification procedure for the Schizosaccharomyces japonicus Bqt1-Bqt2 complex, which is closely related to the S. pombe Bqt1 Bqt2 complex.
A purification procedure for LPPC has not been described as yet and, therefore, we decided to develop a protocol for this purpose, comprising three main steps: (1) dissecting LP from colonic tissue; (2) releasing LP cells by a short 15-min collagenase digestion; (3) isolating LPPC by positive immuno-magnetic selection using the distinctive expression of CD54 (ICAM-I) on LPPC.
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Lysates were obtained from TAP IKKβ cells (heavy) and TAP cells (light) mixed at an equal ratio, before being subjected to a sequential purification procedure as outlined in Supplementary Figure S1(C).
That is, the process is complicated, consisting of a two-step, two reaction-vessel followed by a multiple purification procedure including HPLC purification of the intermediate radiolabeled compound.
These symptoms appeared 3 days after she experienced an accidental needlestick while working with material from a vaccinia virus (strain WR)–infected cell culture during a virus purification procedure.
Using an original purification procedure, we identified a 20 kDa protein that is strongly expressed in chicken gizzard smooth muscle.
Highly abundant proteins are likely to have larger fluctuations in their expression levels and, therefore, if one is utilizing a network deduced from an affinity purification procedure, care must be taken when interpreting the results.
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