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Briefly, 1×10 DF1 cells transfected with RCAS plasmids and producing RCAS viruses, were injected into the brainstem or cerebral cortex of neonatal Ntv-a pups (postnatal day 2 4) in 1 μL volume using a custom Hamilton syringe.
"She's just a pup".
What was a pup tent, exactly?
I know, because I raised him from a pup.
"I got a pup tent, too, you know.
It was a pup that had not been dead long.
Photograph: Diego Azubel/EPA 8.23pm BST I was sold a bit of a pup there.
Every senior Lib Dem knows they have been sold a pup by Cameron".
I preached the Big Tent, but you've got a pup tent, boy.
Skye is such a big baby, vented a pup named True about a rival.
Within a year, "A Pup Just for Me; a Boy Just for Me" was completed.
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