The nanocomposite was prepared by modifying a published procedure [10].
(A): 3,3′-dmbp was prepared by deamination of 4,4′-diamino-3,3′-dmbp 4,4′-diamino-3,3′-dmbp 4,4′-diamino-3,3′-dmbp 4,4′-diamino-3,3′-dmbp
Materials and methods: Zn II Pcs were synthesized according to a published procedure.4 The Zn II Pcs were radiolabeled with 131I by using iodogen method.
Commercially available belatacept (Nulojix®, Bristol Myers Squibb) was conjugated to p-SCN-Bn-DOTA (S-2- 4-isothiocyanatobenzyl -1,4,7,10-tetraazacyclododecane S-2- 4-isothiocyanatobenzyl -1,4,7,10-tetraazacyclododecaneed S-2- 4-isothiocyanatobenzyl -1,4,7,10-tetraazacyclododecane
The bifunctional chelating agent p-SCN-Bn-DOTA was successfully conjugated to belatacept under aqueous conditions by reaction with lysine amino groups of the protein according to a published procedure [14].
The serum sensitivity of the BbCRASP-2 mutant was measured using a published procedure [16].
The lysate was treated with micrococcal nuclease according to a published procedure [46].
To test whether VHL can ubiquitinate p160, we conducted in vitro ubiquitination assays following a published procedure [28], [29].
The insertion mutants were constructed in JR32 using allelic exchange, according to a published procedure [36], [37].
Based on a published procedure [41], we sequenced PCR products using BigDyeV3.1 Terminator Kit for 3730XL (Life Technologies) and assembled the high-quality sequences into the complete cp genome using the Phred-Phrap-Consed package [42], [43].
using a published procedure [ 25].
