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All patients underwent a methacholine bronchial provocation test, and AHR was defined as a provocative concentration causing a 20% fall in FEV1 (PC20) inferior or equal to 16 mg/mL.
The asthmatic group was required to have a provocative concentration causing a 20% drop in FEV1 < 8 mg/mL on histamine challenge.
In the asthmatic group, increased bronchial hyper-responsiveness to methacholine, with a provocative concentration causing a 20% fall in FEV1 <8 mg/mL, was required.
In addition, a provocative concentration causing a 10% drop in FEV1 was determined in both patients with asthma and normal subjects.
Bronchial hyperresponsiveness is present when a 20% fall in FEV1 is obtained with a provocative concentration of histamine (PC20) of 8 mg/ml or less.
Patients with BHR (n= 34), i.e. a provocative concentration causing a 20% fall in FEV1 (PC20) methacholine bromide ≤39.3 mg/ml, were included for further analysis.
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All subjects showed a normal airway response to methacholine (provocative concentration leading to a 20% fall in FEV1 (PC20FEV1) > 8 mg/mL).
To examine for tachyphylaxis to methacholine at 24 h and to use these data to assess the repeatability of the provocative concentration of a substance causing a 20% fall in FEV1 (PC20) for methacholine and to obtain statistical power calculations.
Furthermore, although a correlation of the PC40Rint, EI with the classical provocative concentration causing a 20% fall in FEV1 (PC20FEV1) has been reported (17), data on agreement are presented for the first time in this study.
LS Mean = least squares mean; CI = confidence interval; FENO = Fractional exhaled nitric oxide; AMP PC20 = AMP challenge provocative concentration causing a 20% fall in FEV1; FEV1 = forced expiratory volume in one second.
b = unstandardized regression coefficient, FEV1 forced expiratory volume in one second, PC20 provocative concentration causing a 20% fall in FEV1, AMP adenosine-5′-monophosphate, ECP eosinophilic cationic protein, NO nitric oxide, ppb parts per billion, * variable log-transformed.
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