Sentence examples for a protocol to express from inspiring English sources

DOI: http://dx.doi.org/10.7554/eLife.04489.003 To study Kar3 motors at the single molecule level, we developed a protocol to express and purify full-length kinesin-14 heterodimers from Saccharomyces cerevisiae using affinity tagged Cik1 and Kar3 fused COOH-terminally to a HaloTag that served as covalent attachment site for the fluorescent dye tetramethylrhodamine (TMR).

As the web becomes increasingly social, having an standard protocol to express this from website to website is very important.

We used a lentiviral transduction protocol to express shRNA oligos directed against CRN5 in human WiDr and DLD-1 cells.

As a prerequisite for these experiments, we designed protocols to express and purify both the cytosolic domain of FeoB (NFeoB) and full-length FeoB from Escherichia coli BL21 in purity, quantity and quality needed for EPR studies.

The same protocols to express and purify human Bcl-2 protein were followed but a Source Q15 column was used in the second purification step and protein was eluted in 25 mM Tris, pH 8.0, with NaCl gradient.

The same protocols to express and purify human Bcl-2 protein were followed.

The purpose of the Cuyahoga County Anti-Poaching Protocol is "to express the commitment of the participating communities that they will not actively pursue or encourage the re-location of a business that has not indicated that it is considering a move from its current location in another participating community".

We have used this improved purification protocol to rapidly express, purify and analyze MMP12 mutant proteins.

The same protocol was used to express and purify human thioredoxin although expression was at 37 °C, and the cells used were E. coli BL21 (DE3) from Invitrogen.

This protocol was also used to express the CGRP receptor in Cos 7 where appropriate.

This protocol has been successfully used to express these receptors (Qi et al., 2008).