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Genomic DNA was extracted from the collected specimens using a protocol established for tissue banking.
Genomic DNA and RNA were then extracted from the ground tissue samples using an EZNA DNA/RNA Isolation Kit and a protocol established for human tissue (Omega bio-tek, R6731-02).
After frozen tissue was ground under liquid nitrogen using a Retsch mixer mill 200 (Retsch Limited, Leeds, UK), genomic DNA and RNA were extracted as above using a protocol established for human tissue.
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Synaptosomes were prepared from hippocampi using a volume-adjusted protocol established for whole brains.
"This is a matter involving protocols established for the interaction between committee staff and the CIA," said spokesman Jay Carney.
A protocol was established for board communication with Calpers staff members so that employees would no longer be whipsawed by the board members' competing interests.
A clear protocol was established for students to confidentially seek help and for staff and faculty to assisting students in distress.
INTERVENTION: Patients were randomly assigned to receive zidovudine or placebo initially; a cross-over protocol was established for patients receiving placebo who had disease progression.
The assembly environment and sustainability committee report calls for a protocol to be established for developers to pass benefits on to communities and prepare a detailed target for renewable energy.
A dedicated sample preparation protocol was established for the analysis of cell cultures.
A constant ex vivo CPM protocol was established for the performance of experiments.
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