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Mutant Pump contains a proprietary compound called Hyperox, which pushes the body's nitric-oxide production "past all previous limits," according to its marketing material.
ENERGI-F704 was a proprietary compound generously provided by Energenesis Biomedical Co., Ltd.
The multi-step screening protocol was applied on a proprietary compound collection and a diverse commercial compound collection.
A proprietary compound library containing 5315 structurally diverse and drug-like compounds was used in our in silico screening procedure.
The longevity study diets were purchased from Dyets, Inc. (Bethlehem, PA, USA), and SRT2104, a proprietary compound, was provided by Sirtris Pharmaceuticals, Inc. (Cambridge, MA, USA).
In the present study, ENERGI-F704, a proprietary compound identified from bamboo shoots extract, exerts AMPK activation activity in human microglial cell model BV2.
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For comparison, we have used the same proprietary compound collection (a diverse subset of 5315 drug-like compounds) as in our previous study.
Naproxen and a proprietary Novartis compound were converted into nanosuspensions through wet media milling and dried onto a mannitol based substrate using spray granulation.
Total RNA (500 ng) was labelled using terminal deoxynucleotidyl transferase (TdT) in the presence of a proprietary biotinylated compound using the Ambion whole transcript Expression kit and the whole transcript Terminal Labelling kit (Affymetrix UK Ltd., High Wycombe, UK), following the standard protocols.
Total RNA (500 ng) was labelled using terminal deoxynucleotidyl transferase (TdT) in the presence of a proprietary biotinylated compound using the Ambion whole transcript Expression kit and the whole transcript Terminal Labelling kit (Affymetrix UK Ltd., High Wycombe, UK), following standard protocols.
To test the effects of the proprietary compound SRT2104, 6-month-old male C57BL/6J mice were placed on a standard AIN-93G diet (SD) supplemented with SRT2104 (100 mg kg−1 bodyweight) for the remainder of their lives, which yielded serum concentrations of 261.8 ± 27.0 and 435.7 ± 75.6 ng mL−1 in the morning and evening, respectively.
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