Each researcher then coded a proportion of the transcripts, and coding was checked by another researcher.
However, an alternative explanation is suggested by the fact that a proportion of Zfy1 transcripts do code for the full-length acidic domain.
Analytical rigour will be maintained by using a second researcher to code a proportion of the transcripts to test for reliability within the coding framework.
Complimentary DNA analysis showed aberrant splicing resulting in a premature stop codon and retention of intron 1 in a proportion of mutant transcripts, confirming the loss-of-function effect of the variant.
Approximately 56% (13,052,970) of the raw reads could be mapped back to the final assembly with a mean depth of coverage of 338.47 ± 6069.16 reads per sequence, although a proportion of assembled transcripts exhibited low read support.
The percentage of reads mapped uniquely to transcripts sequences, as a proportion of total transcript mapped reads, was 38.49%, 54.93% and 33.83% for the conditions 48 h glucose, 24 h straw and 5 h glucose respectively (Additional file 5).
Haerizadeh et al. [ 13] identified transcripts expressed selectively in mature pollen of soybean (Glycine max) and revealed that the transcriptome had a high proportion of transcripts involved in signalling, transcription, heat shock response, transporting and the ubiquitin/proteasome pathway.
Thirty percent of all Drosophila genes matched by our ESTs were matches by both multiple singletons and a contig, demonstrating that a sizable proportion of transcripts were sequenced as multiple, independent pieces.
DIANA Cluster 6, comprised of transcripts that remained elevated and stable across a torpor bout, contained a higher proportion of transcripts with Motif 2 (50.8%, Figure 6D) relative to the other DIANA clusters (8.5 39.8%, Figure 6D) and the control, non-significant transcripts (21.1%, NS in Figure 6D).
There was concern that defraying the costs to the physicians might result in reduced transcription and a lower proportion of transcripts on the EMR.
