We would like to thank Dr Olga Kurkina for help with participants' recruitment, Mrs Marina Korobova for performing a proportion of bacteriological work, Ms Olga Ignat'yeva and Ms Svetlana Mironova for performing molecular work on a proportion of isolates.
A proportion of isolates could not be retrieved for genetic analysis.
For combination resistance patterns, results are reported as a proportion of isolates tested to all of the classes of drugs in the specific combination.
†Not all isolates were tested for each second-line drug clasecond-line drugeption of the RepubliclassKorea SRL), so results are reported as a proportion of isolates tested to the specified class of drugs.
For each state in each study year, we calculated a proportion of isolates that were defined as either USA300 or USA100 to account for variable sample sizes in states over years.
In a proportion of isolates (N = 124), genotyping results were validated by using both capillary electrophoresis and manual electrophoresis for PCR fragment separation, and again, no discrepancies were found between VNTR loci copy numbers in strains isolated from the same patient at different time points.
Whole genome high throughput sequencing is probably the ultimate technique for microbial genotyping but even sequencing a proportion of isolates evolving in a clonal population structure such as MTB and subsequent bioinformatic analysis [ 31] will reveal SNPs uniquely associated with specific genotypes and will contribute to the optimization and interpretation of SNP screening assays.
Since 2005, a proportion of isolates exhibiting decreased susceptibility and high-level resistance to ciprofloxacin have been tested for resistance to azithromycin by Etest (AB Biodisk, Solna, Sweden), using drug-sensitive strains of S. Typhi and S. Paratyphi A as controls.
All cryopreserved cells and a proportion of freshly isolated cells were read on a FACSCalibur flow cytometer (Becton Dickinson Immunocytometry Systems, MA, USA) and analysed using Flow Jo software (Tree Star, Oregon, USA).
Whilst the ET programme successfully engaged a proportion of socially isolated OA, most were already socially active to some degree: "We had to work with organisations that had experience and credibility working with older people.
