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As a proof of specificity and efficiency, we show that less than 2% of reads generated by Motorplex are off-target, in comparison with >12% of WES.
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However, our data with irradiated cells demonstrate that such a control is not an adequate proof of specificity.
As an important proof of specificity of PAB binders, we performed cell-surface competition binding assay using LNCaP cells.
The sample amplicons were harvested and sequenced, and functioned as a proof of concept, evaluated the specificity of the Gut Microbiotassay by further elucidating the components of the gut microbiota.
It was a proof-of-concept issue.
Additional proof of specificity was obtained with a dimer containing d-Glu.
Another proof of specificity of the PSMA-binding 68Ga complexes was obtained through the blockade of the high kidney and spleen uptake values with co-injected 2-PMPA (Figure 3b), indicating that the uptake was mainly mediated by specific binding to physiologically expressed PSMA in those murine organs [44 47].
Western blot analysis (see below) of macrodissected material of cryptal epithelial cells and lymphoid cells was performed for further evaluation and proof of specificity of the newly designed antibody.
As a proof of concept for their utility as broad-specificity caspase-cleaved substrate detectors, the NEAs were used to precipitate proteins from lysates of cells induced to undergo apoptosis in the presence or absence of caspase inhibitors.
A proof of concept.
It is also a proof of principle.
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