Sentence examples for a prolonged extension from inspiring English sources

The final cycle had a prolonged extension at 72 °C for 5 mins.

The final step was a prolonged extension of 5 min at 72°C.

A prolonged extension step was carried out at 72°C for 7 minutes.

This, in turn, leads to the second novel feature of our strategy, a prolonged extension step at the end of the first PCR.

The reactions were performed in thermal cycler (MJ Research, CA, USA) using the following profile: one cycle of 3 min at 94°C, 38 cycles of 30 sec at 94°C, 30 sec at 50°C, 55°C or 60°C and 45 sec at 72°C, followed by a prolonged extension of 5 min at 72°C.

We overcame this problem with a final and prolonged extension step, so that all of the partial products are converted to full-length products.

The strategy relies on the use of a limiting concentration of one of the flanking primers (reverse or forward) along with the normal concentration of mutagenic primer, plus a prolonged final extension cycle in the first PCR amplification step.

Amplification was carried out in a Mastercycler gradient (Eppendorf) with denaturation at 95°C for 5 min; 35 cycles with denaturation at 95°C for 30 s, annealing at X°C (optimized annealing temperature Table 3) for 45 s and extension at 72°C for 1 min, followed by prolonged extension at 72°C for 10 min and a short storage at 4°C.

Concerning the distribution pattern of the neurite lengths, the population of long-neurite-bearing cells was much smaller than the population under optimal conditions, whereas the population of short-neurite-bearing cells decreased upon a transient (1 h) second stimulation, indicating that sustained stimulation was required for the prolonged extension of the neurites (see Figure S2D,G,I).

The PCR cycles consisted of 98 °C 30s,15 cycles of 98 °C 10s, 65 °C 30s, 72 °C 30s, followed by prolonged extension for 5 min at 72 °C.

However, intracapsular pressure formed by CRIF compromised femoral head circulation, and prolonged extension and internal rotation position on the fracture table reduced the blood supply to the femoral head, what's more, the repeated forceful manipulation increased the risk of AVN [ 6].