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The photocurable resin is spread on ice surface and then solidified by a projection image.
For each field of view, stacks of 2 images with a Z step size of 0.2 mm were collected and a Metamorph 3D reconstruction tool was used to create a projection image.
It was possible to obtain an isotropic pixel resolution of 7.5 µm using a camera binning mode of 2 by 2 pixels resulting in a projection image of 1000×2000 pixels.
Both spiders were scanned with 66 kV and 133 µA at a 4.3 fold optical magnification (Xradia MicroXCT, Pleasanton, California), resulting in a projection image pixel size of 2.5 µm for the spider fixed 9 days after ecdysis and 2.0 µm for the other, slightly smaller, spider.
(A ) Projection image (left); Fourier transform (center); Fourier synthesis map (right).
(A ) Projection image of a vesicle reconstituted with F1Fo ATP synthase.
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(A ) Projection images of tomographic z-slices containing the whole single-layered 2D crystal were processed to determine unit cell parameters and crystal symmetry.
(A ) A maximum projection image from a movie of an actin filament tethered to a glass surface via a single α-actinin molecule where the tethering position about which the filament swivels is visible as a constriction point.
A common practice is to acquire a single projection image, using a very low electron dose, before a tilt series is collected, and then compare it to an image taken after the series is complete.
Since mammography is a projection radiography technique, it represents a 2D projection image of a 3D object.
For instance, a bright region in a photoacoustic projection image (few of them are indicated by arrows in Fig. 5) can either mean an enhanced local light absorption or that an absorbing structure is oriented along the projection direction.
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CEO of Professional Science Editing for Scientists @ prosciediting.com