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The only way of producing good alignments in such situations is to force a program to align certain known homologies to each other.
MAPGAPS [ 63], a program to align sequences to a hierarchical set of profiles, was used to generate the final core domain alignment.
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It has also been applied to real data as a secondary program to align reads rejected by primary programs [ 17].
That year, the state also adjusted its drug testing program to align it with a uniform program developed within the industry.
Using the BLAST program to align the EST database for the SFAR4 (At3g48460) sequence, full-length SFAR4 cDNA was identified.
We looked for conserved miRNA* in both libraries using BLASTN program to align all Solexa sequences against the hairpin sequences with mature miRNA region masked.
We also ran NCBI bl2seq program to align the two regions (not shown).
We also ran the NCBI BLAST2seq program to align the two regions (not shown).
We used BLAT program to align brain and cell line contigs.
The ability of the programs to align a specific block was estimated by calculating the block column score, (BCS) = mean column score in the block: <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0018093.e002.PNG" class= inline-graphic"/> In this case, the block column scores are not weighted by the number of sequences in the block.
SOMA (Nagarajan et al. 2008) uses dynamic programming to align in silico -digested contigs to an optical map.
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