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The hydrolysis of triolein used as test reaction was performed following a procedure adapted from Rocha et al. (1999).
A tenfold AuNP concentrate was processed under an N2 atmosphere to assess the activated partial thromboplastin time (aPTT) using a procedure adapted from our previous report [17].
For the subcellular and PSD fractionation, five mouse brains were used in a procedure adapted from Huttner et al. [49].
Mammalian-cell-derived PUF60 and U2AF65/35 were expressed in 293E cells transiently transfected with pTT3-HisPUFS or pTT3-HisU2AF35 in a procedure adapted from a published method [60].
Hemichannel activity was measured using a procedure adapted from Orellana et al. (Orellana et al., 2009).
Nuclear extracts were prepared using a procedure adapted from Lee et al. (Lee et al., 1988).
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Seventy two hours post infection the cells were harvested by centrifugation and nuclear extracts were prepared by a procedures adapted from Schreiber et al. [63].
Routine care of tracheal cuff was managed according to an internal procedure adapted from the Société de Réanimation de Langue Française recommendations [ 19].
The scenarios are developed and selected using a six-step procedure adapted from the formative scenario analysis (FSA), shown in Figure 1b.
Kidney triglycerides were extracted from 50 mg frozen corticomedullary kidney sections using a chloroform/methanol (2 1) extraction procedure adapted from previous studies (Frayn and Maycock, 1980; Nogueiras et al., 2007).
Figure 4 presents a schematic of the overall procedure (adapted from Georgopoulos et al. 2005).
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