Herein, we used RNA-Seq platform to characterize the transcriptomic response to low temperature by comparing different transcriptome of Chrysanthemum nankingense plants and subjecting them to a period of sub-zero temperature, with or without a prior low temperature acclimation.
Here, the RNA-Seq platform based on Illumina NGS technology was used to characterize the transcriptomic response to low temperature by comparing the different transcriptome of C. nankingense plants subjected to periods of sub-zero temperature with or without a prior low temperature acclimation, with a view to gaining a deeper insight into the molecular basis of this physiological adaptation.
Cold receptors respond to sudden cooling with a transient increase in discharge frequency (called the dynamic response) that is directly related to the prior temperature and the magnitude and rate of the temperature decrease.
A number of perennial and biennial plants (bulbous plants, beets) require a prior period of low temperature before they flower.
When temperatures increase gradually or when plants experience a prior exposure to moderately high temperatures, changes in gene expression ensue (Larkindale et al. 2005; Hannah et al. 2006; Larkindale and Vierling 2008), leading to greater thermotolerance.
To avoid post-curing effects in the adhesive, a constant load was applied to the bar in a pull-compression configuration prior to temperature increase.
Indeed, a simple modification to the conventional tSMS DNA template preparation procedure, involving a modified denaturation temperature prior to template 3' poly-A tailing, increased the efficiency of endogenous sequence recovery, suggesting that the efficiency of tSMS could be further improved for sequencing ancient DNA extracts [ 10].
The Eulerarian-Eulerian multiphase model system described herein is a fluidized bed of sand externally heated to a predetermined temperature prior to introduction of agricultural biomass.
Because the acid mixture is brought rapidly to a boil, it is important to make sure that the cooling water has reached a stable temperature prior to heating the sample.
We also showed that this process could be improved by performing prehydrolysis at a higher temperature prior to SSF.
