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For more complex tissue of bone segments shot through with vessels, we hypothesize that an altered strategy might be effective: starting from in vitro tissue engineering for a thin sheet of primary product, then transferring to in vivo cultivation with a self-expanding procedure activated by interweaving metal springs of shape memory alloy (SMA).
A 30 × 40 mm oval bovine pericardial sheet functioning as a primary closer of the septal defect was approximated to the ventricular septum and secured.
Examination of cells by TEM clearly showed that the primary keratinocytes were highly adherent, forming a confluent sheet of cells, extensively connected through dense plaque containing desmosomes.
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