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Furthermore, VACV-infected S2 cells did not support the replication of a transfected plasmid, which occurs in mammalian cells and is dependent on all known viral replication proteins, indicating a primary restriction of DNA synthesis.
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The goal of the game is to trade a ball token for points by placing it in an opponent's net, with the primary restriction being that you can't handle the ball.
The primary restriction for accurate ploidy analysis in this case is probably the experimental protocol, which calls for a standard amount of DNA to be assayed.
NlaIII (New England Biolabs, Ipswich, MA) was used as the primary restriction enzyme and DpnII (New England Biolabs) was used as the secondary restriction enzyme.
Mechanically shearing would have the advantage that the chance of capturing a fragment is improved, because some of the secondary restriction sites are too close to the primary restriction sites.
Currently, the technology and development state of the single-site ECMO support is the primary restriction that is limiting wider use.
Of the 3 primary restriction fragment polymorphism (SAG2) genetic types of T. gondii, investigators have primarily found the type 1 organism in southern Brazil (7) and have identified it from chickens (8, 9 ).
One of the primary restrictions of the GARCH models is that it forces a symmetric response of volatility to positive and negative news.
Crown Prince Abdullah replied that the presence of the two holy shrines on Saudi soil were the "primary restrictions" in making changes in the kingdom.
Primary restrictions guide the selection of entities and the set of conservation and balance equations needed to formulate an augmented entropy inequality (EI).
Because of the given non-causality of secondary analyses and the restriction in variables, a primary analysis might overcome these restrictions.
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