Sentence examples for a primary passage from inspiring English sources

Features of the disease and PrPres molecular features after a primary passage in C57Bl/6 mice have previously been described for two of the H-BSE and for the classical BSE isolate [26], [27].

Resurrection of subsequent cultures starts with a cryopreserved primary passage with culturing performed to expand the culture and/or generate cells for DNA isolation.

Might a small number of 'activated' or 'aggressive' FLSs present in a primary culture (passage 1) overgrow other elements and appear as a major population in later (passage 4) cultures?

The distribution of different PrPSc types in the rodent brain at primary passage can provide a means of identifying different strains in wild type (22, 28 ) and transgenic mice (32 ).

First, we isolated primary (passage 2) mouse embryonic fibroblasts (MEFs) and transduced them with a retrovirus encoding the Cre recombinase (pBabe-Cre).

However, in the case of the Suffolk sheep, the incubation period for the TgBoPrP mice was 693.8 d in the primary passage and 457.7 d in the second passage.

All of the 89 samples that were classed as non-culturable did not show any growth in tissue on the primary passage.

To detach the primary passage cells, chondrocytes were digested using 0.05% trypsin:EDTA (GIBCO), and then counted using a hematocytometer.

For subpassages, 10% brain homogenates from Tg110 mice collected from primary passage were used as inocula.

Once the primary (passage zero) cells reached confluence (20 days), the FBS concentration was reduced to 5%.

With the exception of Figure 2D, all data for DNA-PKcs -/- cells were from primary (passage 1) cells.