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International Journal of Refrigeration doi 10.1016/j.ijrefrig.2009.01.013 presented a hybrid approach for providing control of refrigerant flow distribution in evaporators that involves the use of small balancing valves in each circuit along with a primary expansion device to control the overall superheat from the evaporator.
The presently described P. t. altaica/ P. t. virgata affinity with P. t. corbetti is consistent with, and may be best explained by, a primary expansion of tigers from China (P. t. corbetti) via the Silk Road through the Gansu corridor (route C) directly into the Tarim Basin/Central Asia (P.
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For mice lacking CD80/CD86, one study reported an impaired primary expansion of MHV-68-specific CTLs [42], while in another study, the frequency of MHV-68-specific CTLs was unaffected by the lack of CD80/CD86 [43].
Our data demonstrate a dramatic decrease in the primary expansion of naïve T cells when cultured with HIV-1-exposed DC.
For primary expansion of Treg, higher proliferation rates were achieved with beads as compared to platebound anti-CD3+ soluble anti-CD28 mAb (Figure 4A).
These results are in agreement with those reported previously by numerous investigators that CD4+ T cells are dispensable for the primary expansion of CD8+ T cells and their differentiation into cytotoxic effectors [2], [3], [5].
Using the MHC class II tetramers, the frequency of antigen-specific CD4 T cells after primary expansion was similar in ICOS /– and ICOS+/+ mice.
DOI: http://dx.doi.org/10.7554/eLife.07486.015 10.7554/eLiFigure86.016 Figure 7 figure supplement 2. OX40L- and 4-1BBL-mediated costimulation is dispensable for primary expansion of MCMV-specific CD8+ T cells.
ICOS B7h interaction could be important in amplifying the primary expansion and promoting the differentiation of effector T cells, perhaps Th2 cells and Tr1 cells.
In contrast, our analysis using MHC class II tetramers of the primary expansion of endogenous, polyclonal CD4 T cells revealed no defect in ICOS knockout mice.
At this time, cells were removed to determine gene expression patterns after primary expansion (P0), used for in vivo experiments, or cultured for an additional 12 to 14 days to provide first subculture (P1) cells.
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