The tissue explants were transferred to a primary culture dish and supplemented with low glucose Dulbecco's Modified Eagle Medium (DMEM; Life Technologies Pty Ltd., Australia) containing 10% fetal bovine serum (FBS; In Vitro Technologies, Australia) and 1% penicillin/streptomycin (P/S; Life Technologies Pty Ltd., Australia) at 37°C in 5% CO2.
I have to find demotic liquids for under a tenner to go with these devious luxury goods, and frankly, contemplating my primary dish of beetroot, blood orange and asparagus, I struggle.
When you choose the primary dish, make sure it's something that could go along with the side dishes.
Main courses, generally rustic dishes of a primary ingredient accented by a secondary flavoring, include tender boiled beef ($4) that is a perfect vehicle for a mildly sharp horseradish sauce, and cabbage, boiled until almost sweet and stuffed with ground pork ($4).
Olive oil is a primary ingredient of many Italian dishes.
Cook dishes that do not rely on meat as a primary protein ingredient, such as noodles with peanut sauce.
The primary reflector dish has a focal length of 200 mm and is a focal with the secondary inverse reflector which has a focal length of 70 mm.
Briefly, lysates from a 10-cm dish of primary keratinocytes were isolated in the presence of cycloheximide (Sigma-Aldrich, St . Louis MI, 50 μg/ml) and subject to limited RNAse digestion (10 units) for 45 min at room temperature.
One xenograft piece was seeded into a culture dish to establish the primary culture cell line, named XUNE2; one was used to continue generating transplants; the final piece was fixed for further studies.
Mr. Scheinzeit works from a palette of bright primary colors and bright primary flavors, most vividly on display in a winning dish called Cape Cod sea scallops.
