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Five exemplary pulses were implemented into a PRESS sequence and validated by acquiring images of a water oil phantom and lactate spectra at TE = 144 ms.
MRI (magnetic resonance imaging) was performed with a 1.5 T system; spectral acquisition was performed with a single-voxel technique and a PRESS sequence.
Using a PRESS sequence with parameters: TR, 10 s; TE, 9 ms and 64 averages, localised 1H MRS from the voxels were collected.
A PRESS sequence with a TR/TE 2500/22 ms was used and the LCModel was used for analysis.
For H-MRS measurement of intramyocellular lipid (IMCL), single voxel (2 cm) spectra localised to the right quadriceps muscle were acquired using a PRESS sequence (TR 5,000 ms/TE 30 ms) [ 32].
Spectra were acquired using first-order iterative shimming, a PRESS sequence with relaxation time/echo time (TR/TE) 2,000/35 ms and 64 signal acquisitions during free breathing.
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Data were acquired using a standard PRESS sequence with an echo time of 30 ms, a relaxation delay of 2,000 ms and 176 transients.
Bilateral single-voxel spectra were acquired using a standard PRESS sequence to provide a metabolite profile from three brain regions of interest (ROIs): basal ganglia and frontal and temporal lobes.
The MEGA-PRESS sequence is able to highlight the GSH signal by adding two editing pulses with a normal PRESS sequence.
First, a standard PRESS sequence (TR = 3 s, TE = 68 ms) was used to acquire an unedited spectrum with 32 averages.
In brief, a voxel of interest was placed over the hand motor cortex in session 1 and the visual cortex in session 2. A standard PRESS sequence was acquired to assess the creatine and NAA line widths, and a MEGA-PRESS sequence was then acquired to allow simultaneous spectral GABA editing, three-dimensional voxel localization, and water suppression [ 23].
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