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The content of SOD proteins was calculated by a prepared standard curve.
The rate of formation of 2-nitro-5-thiobenzoic acid is proportional to the glutathione concentration and was monitored at 412 nm over the period of 2 min and the size of the GSH pool was calculated from a prepared standard curve.
The absorbances obtained were extrapolated from a prepared standard graph.
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The l-alanine-aminopeptidase activity was calculated using a previously prepared standard curve with a 4-nitroaniline concentration range of 0.16 to 24 mg/L (Merck, Darmstadt, Germany).
The concentration of the total ions present in the tested suspension was indicated by the records of electric current potential, which can be read from a previously prepared standard curve of electric conductivity of known concentration of known electrolyte [33].
The real-time PCR measurements were carried out using an ideally prepared standard curve.
Initial results indicate, although not yet conclusive, that a 57Co-MES catalyst can be prepared from the industrial prepared standard Co catalyst by an additional simple incipient wetness impregnation procedure.
The prepared standard was kept in a refrigerator at 5 ± 3 °C for not more than 3 months.
The design of railway lines under the current standard STN 73 6360 [1] including the comparison of railway line parameters to the European Standard EN 13803-1 anewa new proposal in accordance to the new prepared standard STN 73 6360-1 [2] of Slovak Railways (ŽSR).
The solution was prepared by accurately adding appropriate volume of each of the prepared standard solution.
After 30 min incubation at 37°C, the absorbance at 450 nm was measured using a microplate reader (Biorad, USA).The SOD activities were calculated by prepared standard curves.
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