The first is a preferential targeting of those elements to the regions harboring those genes.
We found a marked inhibition of sphere formation by the combination of chemotherapy and the Chk1 inhibitor, thus confirming a preferential targeting of the clonally expanding cells.
Moreover, the overlap of OATP1C1 expression with Aquaporin 4 (Roberts et al. 2008) suggests a preferential targeting of the transporter to astroglial end feet of the blood brain barrier.
Whereas P42-TAT could be detected in different neuronal populations (NeuN cells Figure 3) or cilia cells (Additional file 1: Figure S2), but also in astrocytes (data not shown), we could not identify a preferential targeting.
Our results indeed reveal a preferential targeting of the deaminases to defined regions of the genome that is not dependent on cofactors but is rather based on accessibility, with structural features of the DNA at the promoter of actively transcribed genes being the key determinant.
Following 48 h of incubation with T-DM1, HER2-positive breast cancer cell lines like SK-BR-3 and BT-474 dose-dependently lost their clonogenic potential, which suggests a selective depletion of clonogenic cells and thus a preferential targeting of CSC by T-DM1.
