Sentence examples for a predetermined time point from inspiring English sources

However, in study 1 cattle were culled at a predetermined time point post challenge or, in both studies, at a predetermined end point when the development of BSE was not expected in surviving animals.

The cells (3 × 105) were seeded on the scaffold which is immersed in the media and allowed to incubate for a predetermined time point.

As this was a retrospective study without a predetermined time point of TCD and/or other imaging evaluation of the patient, the exact onset of vascular alterations and/or stroke might have been missed.

Polymerization was terminated at a predetermined time point to yield different molecular weights by exposing the reaction mixture to air.

At the predetermined time point, an aliquot of 20 μl volumes were removed from each test suspension and plated on SDA for determining the colony forming units.

As shown in Fig. 8, the DNA percent content at the predetermined time point maintained above 95% during 20 days.

Myofibroblasts were collected from the gels at the predetermined time point using collagenase 5 mg/ml (Sigma, St . Louis MO) containing cycloheximide (100 µg/ml) and collected by centrifugation.

Then, at each predetermined time point (0, 4, 8, 24, 48, and 72 h after drug administration), six mice were sacrificed.

At each predetermined time point, the ND-AMC solution was centrifuged for 20 min at 14000 g, the supernatant was recovered, and the pellet was resuspended in 1 mL of PBS solution and incubated at 37 °C until the next time point.

For the DNA/ALP test, at predetermined time point the hydrogels were washed twice with ice-cold PBS, transferred to centrifuge tubes containing 300 mL cell lysis buffer (10 mM Tris-HCL, 10 mM NaH2PO4/NaHPO4, 130 mM NaCl, 1% Triton X-100, and 10 mM sodium pyrophosphate; BD Biosciences), and lysed at −4°C for 45 min.

At predetermined time points (0.0, 0.5, 1.5, and 4 h) a sample of the competition solution (10 μL) was added to an EDTA-solution (50 mM in Chelex-water, 40 μL) and subjected to analytical HPLC purification (0.8 mL/min, 0% to 90% B in 17 min).