Supplementary Figure 4 Transcriptional interference can generate a precise threshold, which is required for reliable mono-allelic Xist up-regulation.
Here, we show that there is a precise threshold level of MYC expression required for maintaining the tumor phenotype, whereupon there is a switch from a gene expression program of proliferation to a state of proliferative arrest and apoptosis.
This could explain, at least in part, why it is so difficult to determine a precise threshold concentration that is harmful or safe for each individual (Cetta et al. 2007).
Large doses of vitamin A taken by a pregnant woman also can cause developmental abnormalities in a fetus, especially if taken during the first trimester; the precise threshold for causing birth defects is unknown, but less than 3,000 μg (10,000 IU) daily appears to be a safe intake.
However, as establishing the nature and degree of harm is a notoriously subjective process, it is difficult to develop precise thresholds for what constitutes harm or otherwise, not least as the type and severity of harm depends on the nature of the target.
Choosing appropriate performance metrics and determining precise threshold values for these metrics is not a trivial task and becomes more challenging if the workload pattern is continuously changing [13].
Precise threshold values for diabetic patients and normal controls need to be further explored for the individual I- mIBG variables (HMR, WR and TDS) to define CAN, and these cut-off values may be dependent on patient demographics and acquisition.
As seen in Additional file 2 and Additional file 3, CTen's performance is robust to the precise threshold used for developing the HECS gene databases.
By implementing an adjustable threshold comparator, a precise decision threshold could be established corresponding to pathological levels of the target biomarkers.
