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Given that Trisk 95 and Trisk 51 seem to mutually regulate their expression level and given that triadin null mice exhibit muscle weakness (7), it is tempting to speculate that a precise expression level of the triadins is crucial for normal muscle function (31); an excess of triadin could be as deleterious as a lack of triadin on the muscle physiology.
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For a number of these factors, it is clear that the factor's precise expression level is critical for normal development.
This technique was selected to survey the expression pattern rather than determine precise expression levels, since a high degree of SCGB1A1 variation was expected between different tissues.
This approach also suffers from the difficulty of achieving precise expression levels for gene products because copy numbers of plasmids are variable.
RNA Seq provides unparalleled levels of information about the transcriptome including precise expression levels over a wide dynamic range.
If the modulation of the transgene expression levels by different drug concentration with the bi-cistronic expression vector enables us the precise control of transgene expression, the monitoring of the expression levels of the drug resistance genes in living cells will be ideal.
Nevertheless, Tarnita and Nowak continue to insist that Hamilton's equation is not a precise expression of a biological phenomenon.
The pathway-focused GE Array is a useful way to perform both highly sensitive and precise large-scale analysis of the expression level of various genes [ 8, 9].
Furthermore, the expression level of Oct4 is regulated in a precise manner in ESCs such that an increase in Oct4 expression level leads to differentiation into primitive endoderm and mesoderm, whereas a decrease results in trophectoderm formation [39].
Although the precise relationship between increased activities of PKB/Akt and ERK1/2 by aging and decreased expression of Gβ4 remains unknown, current data represent the first demonstration of a change in Gβ4 expression with age in rat brain and suggest that an age-related alteration in expression level of Gβ4 may, at least in part, play an important role in aging processes.
To determine whether the expression level of CB1 or CB2 receptors dictate cannabinoid-mediated responses in astrocytomas, we sought to use heterologous gene expression technology to drive the expression of these receptors to precise levels in an astrocytoma cell line that normally lacks these receptors.
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